Journal: Frontiers in Microbiology

Article Title: Paving the way or sharing goods?—Interactions between pairs of Staphylococcus aureus and Pseudomonas aeruginosa sequentially isolated from respiratory samples of patients on mechanical ventilation

doi: 10.3389/fmicb.2026.1798383

Figure Lengend Snippet: Relative biofilm formation by monocultures and cocultures in the three media: (A) M1, (B) M2, and (C) M3. Biofilm formation was assessed by the crystal violet assay and the biofilm volume normalized to that of the reference stain Pa01 in each medium. The bars represent an average of 12 culture wells in 96-well plates from two different biological replica performed on different days. Error bars represent standard deviation. *Represent significantly different values ( p < 0.01, Students t -test) between monoculture and coculture. Blue bars represent P. aeruginosa ( Pa ) and red bars S. aureus ( Sa ), crossed bars cocultures.

Article Snippet: Thereafter, the suspensions were diluted to OD 600 = 0.1, and from this suspension 20 μL were added to 180 μL pure media in flat-bottom 96-well plates (standard non-coated microplates, Sarstedt).

Techniques: Crystal Violet Assay, Staining, Standard Deviation